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  • Title: Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps.
    Author: Cardinali G, Pellegrini L, Martini A.
    Journal: Yeast; 1995 Sep 15; 11(11):1027-9. PubMed ID: 7502578.
    Abstract:
    A modified procedure is proposed for chromosomal DNA extraction based on a cell-wall lytic enzyme never applied before in pulsed field gel electrophoresis. Protoplasting efficiency is retained under very challenging conditions for enzyme activity, such as those required for non-Saccharomyces yeasts often characterized by cell walls highly resistant to lysis.
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