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  • Title: Characterization of murine monoclonal antibodies directed against the submembrane protein p17 of HIV-1.
    Author: Niedrig M, Harthus HP, Bröker M, Meloen R, Gelderblom H, Pauli G.
    Journal: Hybridoma; 1993 Aug; 12(4):431-9. PubMed ID: 7503941.
    Abstract:
    Monoclonal antibodies (MAbs) were raised against the gag protein of human immunodeficiency virus type 1 (HIV-1). The reactivity of the selected Mabs with the matrix protein p17 of HIV-1 were investigated in several tests, i.e. ELISA, immunostaining of Western blots, and alkaline phosphatase anti-alkaline phosphatase immunocytochemistry (APAAP). All three Mabs reacted exclusively with HIV-1 and showed specific binding to the virus surface in pre-embedding immuno-electron-microscopy and useful as diagnostic agents. In an "in vitro" cultivation experiment the MAbs showed antiviral activity in concentrations in the range of 25-100 micrograms/ml. No binding region could be defined using overlapping peptides consisting of the p17 protein sequence of HIV-1 in an epitope mapping system and therefore we concluded, that the MAbs recognize a conformational epitope.
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