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  • Title: Development and application of a polymerase chain reaction assay for Mycoplasma synoviae.
    Author: Lauerman LH, Hoerr FJ, Sharpton AR, Shah SM, van Santen VL.
    Journal: Avian Dis; 1993; 37(3):829-34. PubMed ID: 7504919.
    Abstract:
    Mycoplasma synoviae (MS) species-specific primers selected from the 16S rRNA sequence were evaluated by polymerase chain reaction (PCR). The MS primers were MS-1 (5'-GAAGCAAAATAGTGATATCA-3') and MS-2 (5'-GTCGTCTCCGAAGTTAACAA-3'). Analysis of cultures of avian mycoplasmas using the MS PCR indicated 100% specificity and sensitivity: 55 individual isolates of MS tested PCR-positive, and 44 isolates of eight other species of avian mycoplasmas tested PCR-negative. The MS PCR will detect 100 colony-forming units of MS. Analysis of 122 flock data sets indicated a sensitivity for the MS PCR test of 82% and a specificity of 100% as determined by comparison with culture, serology (serum plate test, hemagglutination-inhibition, enzyme-linked immunosorbent assay), epizootiology, and history.
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