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Title: [Immunologic characteristics of monoclonal antibodies to Mycobacterium Bovis and four affinity-purified mycobacterial antigens]. Author: Liashchenko KP, Bobrovnik CA, Gorbenko EV, Kolesnikova IN, Bukhanevich AM, Liashko ED, Komissarenko SB. Journal: Probl Tuberk; 1993; (5):41-4. PubMed ID: 7507592. Abstract: Monoclonal antibodies to M. bovis were obtained and characterized. Affinity-purified mycobacterial antigens were isolated from tuberculin PPD using these antibodies. Comparative immunochemical analysis of four antigens was carried out. Sufficient differences between the antigens were observed in both ELISA and tuberculin skin tests on guinea pigs. Serologically specific for M. bovis 3-1C-antigen was shown to express cross-reacting T-epitopes, in contrast to another specific antigen isolated earlier in the same way using rabbit polyclonal antibodies against tuberculin PPD. None of the specific mycobacterial antigens isolated was immunodominant in the serodiagnostic test for tuberculosis.[Abstract] [Full Text] [Related] [New Search]