These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Rapid microscopical diagnosis of deep-seated mycoses following maceration of fresh specimens and staining with optical brighteners.
    Author: Rüchel R, Margraf S.
    Journal: Mycoses; 1993; 36(7-8):239-42. PubMed ID: 7509452.
    Abstract:
    A fluorescent staining procedure, which used to be recommended for the detection of fungi in skin and nail scrapings, can be adapted for the rapid detection of fungal elements in non-sectioned biopsies or respiratory secretions. The specimens are directly stained with an optical brightener (Blankophor P flüssig, Cellufluor, Uvitex 2B) preferentially after exposure to 20% aqueous potassium hydroxide solution. The procedure can also be applied to Gram-stained microscopic mounts. The high intensity of the fluorescence elicited allows rapid microscopic screening at low magnifications.
    [Abstract] [Full Text] [Related] [New Search]