These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A radioimmunoassay for 3,5,3'-triiodothyronine in chicken serum.
    Author: May JD.
    Journal: Poult Sci; 1978 Nov; 57(6):1740-5. PubMed ID: 751048.
    Abstract:
    A double-antibody radioimmunoassay is described for 3,5,3'-triiodothyronine (T3) in chicken serum. Primary antibody produced in a rabbit had negligible crossreactivity with 3,3,5'-triiodothyronine and thyroxine. The primary antibody diluted 1:1000 bound 50% of a tracer dose of 125I-T3, and a standard curve of 0 to 800 pg/tube was used. Percent binding ranged from 14.1 to 54.1 for a standard curve. Serum volume ranging from 50 to 150 microliter tube did not affect assay results. Coefficient of variation was 2.8% within the assay and 5.5% between assays. The inhibitor 8-anilino-1-naphthalene sulfonic acid was necessary for recovery of T3 from spiked samples. Young chickens had reduced serum T3 concentrations when fed diets containing .1% thiouracil for 14 days when compared with serum T3 concentrations of chickens fed the diet without thiouracil. Feeding .2% thiouracil for 3 days did not affect serum T3 concentration.
    [Abstract] [Full Text] [Related] [New Search]