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  • Title: A simple method for demonstration of eosinophils in frozen sections.
    Author: Furgał A, Litwin JA.
    Journal: Folia Histochem Cytobiol; 1993; 31(4):197-9. PubMed ID: 7511115.
    Abstract:
    Tissue eosinophils can be easily and specifically demonstrated in frozen sections after substrateless incubation with 3,3'-diaminobenzidine (DAB) at neutral pH. Under such conditions, other cells possessing endogenous peroxidatic activity (neutrophils, macrophages) do not stain, whereas staining of mitochondria-rich cells due to cytochrome oxidase activity can be avoided by a short prefixation of sections in glutaraldehyde-containing fixatives. The method can be useful in cases when a rapid screening of tissue eosinophils is required.
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