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  • Title: Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.
    Author: Biała E, Milecki J, Kowalewski A, Popenda M, Antkowiak WZ, Adamiak RW.
    Journal: Acta Biochim Pol; 1993; 40(4):521-30. PubMed ID: 7511312.
    Abstract:
    Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.
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