These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes.
    Author: Parry SL, Holman MJ, Hasbold J, Klaus GG.
    Journal: Eur J Immunol; 1994 Apr; 24(4):974-9. PubMed ID: 7512037.
    Abstract:
    It is widely accepted that extensive cross-linking of surface immunoglobulin (sIg) receptors on mature B cells promotes their activation and progression through the cell cycle. A commonly employed method to maximize receptor cross-linking via anti-receptor antibodies is to immobilize them on tissue culture plastic. We show here that immobilizing monoclonal anti-mu or anti-delta antibodies, which are mitogenic in solution, on plastic abrogates their capacity to induce DNA synthesis in mature murine B cells, even in the presence of interleukin-4 (IL-4). The cells do become abortively activated, as evidenced by up-regulation of major histocompatibility complex class II antigen levels, but subsequently virtually all of them die, manifesting DNA fragmentation characteristic of apoptosis. The induction of apoptosis is abrogated by the inclusion of either IL-4 or anti-CD40 antibodies in the cultures, with the two stimuli acting in concert. We believe that the system represents a polyclonal model of clonal deletion tolerance in mature B cells, such as may be induced under physiological conditions by antigens with repeating epitopes.
    [Abstract] [Full Text] [Related] [New Search]