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  • Title: Matrix-assisted laser desorption/ionization with an external ion source Fourier-transform mass spectrometer.
    Author: McIver RT, Li Y, Hunter RL.
    Journal: Rapid Commun Mass Spectrom; 1994 Mar; 8(3):237-41. PubMed ID: 7513214.
    Abstract:
    Mass spectra of several model oligopeptides (substance P, [Arg8]-vasopressin, tyrothricin, and the B-chain of bovine insulin) have been obtained with a matrix-assisted laser desorption/ionization (MALDI) source and a custom-designed Fourier-transform mass spectrometer (FTMS). The MALDI source is outside the magnetic field in a separately pumped external chamber, and ions are injected through the fringing fields of the magnet and into the FTMS analyzer cell by a long quadrupole mass filter that is operated in the RF-only mode. A large window on the ion source housing makes it easy to point and focus the laser beam onto the sample probe tip. A good quality mass spectrum is achieved for 0.5 pmol of substance P, and the mass resolution for the B-chain of bovine insulin is 19,000.
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