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  • Title: [The determination of the antigenic activity of recombinant virus-specific polypeptides from the herpes simplex virus types 1 and 2 and their use in immunoenzyme analysis].
    Author: Zvonarev AIu, Klichko VI, Shatalin KIu, Kuliakina MN, Karaseva EV.
    Journal: Vopr Virusol; 1994; 39(2):59-62. PubMed ID: 7517084.
    Abstract:
    The structural and nonstructural recombinant proteins of HSV type 1 and type 2 were expressed in E. coli by fusion with cro-beta-galactosidase proteins. These proteins containing amino acid sequences of ICP4, ICP27, ICP47, ICP22, major capsid protein and major DNA-binding proteins of HSV types 1 and 2 reacted in immunoblotting with the corresponding anti-HSV sera from hyperimmune rabbits. The nonstructural recombinant proteins can be used for enzyme immunoassay detection of HSV1 or HSV2 type-specific antibodies in sera from patients with acute HSV infection.
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