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  • Title: Diversity in molecular mass of the common EDTA-soluble antigens of Clostridium chauvoei and Clostridium septicum.
    Author: Hamaoka T, Terakado N, Nakamura S.
    Journal: Microbiol Immunol; 1994; 38(2):149-52. PubMed ID: 7518890.
    Abstract:
    Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of C. septicum, in IFA. In immunoblot analysis with the mAbs, the bands at molecular mass of 35 kDa were found in all C. chauvoei strains, while the bands at 36 kDa were found in 4 of 5 strains of C. septicum. These results indicate that the 35 kDa antigen of C. chauvoei and the 36 kDa antigen of C. septicum possess a similar epitope recognized by the mAb.
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