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  • Title: Production and characterization of monoclonal antibodies against the ribosome inactivating proteins dianthin32 and momochin.
    Author: Porro G, Bonardi MA, Giovanetti E, Lento P, Modena D.
    Journal: Hybridoma; 1994 Apr; 13(2):99-105. PubMed ID: 7519581.
    Abstract:
    Female BALB/c mice were immunized with either dianthin32 or momochin, type 1 ribosome-inactivating proteins (RIPs) derived from Dianthus charyophyllus and Momordica cochinchinensis, respectively. Five anti-dianthin32 and 6 anti-momochin secreting hybridomas were obtained by somatic fusion of lymphocytes with myeloma cell line NS0. The monoclonal antibodies (MAbs) produced were highly specific, as demonstrated by cross-reactivity assays performed with taxonomically related and unrelated type 1 RIPs, and recognized different epitopes of the antigen. The affinity constant of anti-RIPs MAbs ranged between 10(8) M-1 and 10(10) M-1.
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