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  • Title: Suppression of a mitochondrial point mutation in a tRNA gene can cast light on the mechanisms of 3' end-processing.
    Author: Rinaldi T, Francisci S, Zennaro E, Frontali L, Bolotin-Fukuhara M.
    Journal: Curr Genet; 1994 May; 25(5):451-5. PubMed ID: 7521797.
    Abstract:
    We used a genetic approach to study the nuclear factors involved in the biogenesis of mitochondrial tRNAs. A point mutation in the mitochondrial tRNA(Asp) gene of Saccharomyces cerevisiae had previously been shown to result in a temperature-sensitive respiratory-deficient phenotype as a result of the absence of 3' end-processing of the tRNA(Asp). Analysis of mitochondrial revertants has shown that all revertants sequenced have a G-A compensatory change at position 53, which restores the hydrogen-bond with the mutated nucleotide. We then searched for nuclear suppressors to identify the nuclear gene(s) involved in mitochondrial tRNA 3' end-processing. One such suppressor mutation was further characterized: it restores tRNA(Asp) maturation and growth at 36 degrees C on glycerol medium in heterozygous diploids, but leads to a defective growth phenotype in haploids.
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