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  • Title: Generation and characterization of a human monoclonal IgG antibody specific for HLA-B12.
    Author: Siemoneit K, Wölpl A, Wegener S, Ernst M, Sonneborn HH, Goldmann SF.
    Journal: Tissue Antigens; 1994 Jul; 44(1):25-33. PubMed ID: 7526486.
    Abstract:
    We describe here the generation and characterization of a human monoclonal IgG antibody (UL/F14) specific for HLA-B12. The antibody is suitable for complement-dependent lysis on lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells. UL/F14 competes for antigen binding with an HLA-B12 human monoclonal IgM antibody and with a specific alloantiserum. Protein chemistry shows that the monoclonal antibody UL/F14 can precipitate solubilized HLA-B12 molecules.
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