These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Organotypic growth and differentiation of human mammary gland in sponge-gel matrix supported histoculture.
    Author: Baibakov BA, Chipisheva TA, Guelstein VI, Ermilova VD, Polevaya EB, Vasiliev JM, Margolis LB.
    Journal: In Vitro Cell Dev Biol Anim; 1994 Aug; 30A(8):490-5. PubMed ID: 7527285.
    Abstract:
    Blocks of breast tissue obtained during radical mastectomies from 23 patients with mammary gland carcinomas were used for cultivation in native-state, gel-supported histocultures. We show that the human mammary gland can be successfully maintained in this system so that normal epithelial breast structures proliferate and undergo differentiation for several weeks and a well-developed system of ducts and lobules is formed. Using antibodies to individual keratins 17 and 8 we have shown for the first time that ducts and alveoles developing in vitro undergo differentiation into the lining epithelium and myoepithelium in the same way as mammary gland epithelium in vivo. Growth of epithelial structures in vitro is also accompanied by the development of continuous basal membrane.
    [Abstract] [Full Text] [Related] [New Search]