These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Bovine fetal-liver stromal cells support erythroid colony formation: enhancement by insulin-like growth factor II.
    Author: Li Q, Congote LF.
    Journal: Exp Hematol; 1995 Jan; 23(1):66-73. PubMed ID: 7527785.
    Abstract:
    Stromal cells are one of the components of the hematopoietic microenvironment, which is crucial for the proliferation and differentiation of hematopoietic cells. We have obtained a bovine fetal-liver stromal cell line that supports erythroid colony formation in the presence of erythropoietin. The cells are cytokeratin-negative and vimentin-positive, indicating a mesenchymal origin. Furthermore, they have phagocytic activity and show endothelial-like morphology. Erythropoietin at a concentration of 2 mU/mL was sufficient to increase erythropoiesis in the presence of these stromal cells, and the effect could be further enhanced in the presence of physiological concentrations of insulin-like growth factor II. The supportive role of stromal cells on erythroid colony formation could still be observed, although to a lesser extent, when the erythroid precursors were physically separated from the stromal monolayer by an agar layer or with serum-free media conditioned by stromal cells. Ultrafiltration of the conditioned media indicated that the active factors have a nominal molecular weight smaller than 3 kD, which does not correspond to any of the known cytokines with erythroid-cell stimulating activities. Our data suggest that these fetal-liver stromal cells play an important role in fetal erythropoiesis, a function which was previously thought to be played exclusively by fetal hepatocytes.
    [Abstract] [Full Text] [Related] [New Search]