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  • Title: [Enzymatic determination of uric acid in serum with the trinder reaction (author's transl)].
    Author: Prencipe L, Fossati P, Vanzetti G.
    Journal: Quad Sclavo Diagn; 1978 Sep; 15(3):382-94. PubMed ID: 752830.
    Abstract:
    We describe a direct colorimetric method for the enzymatic assay of uric acid. 50 microliters serum aliquots are incubated for 30 min with a diluted hexacyanoferrate solution, to remove interference by non-specific reducing agents. Uric acid is then oxidized to allantoin in the presence of uricase (Aspergillus flavus), with the simultaneous production of hydrogen peroxide; this is measured by a modified Emerson-Trinder reaction, in which phenol has been replaced by sulphonated 2,4-dichlorophenol, a more sensitive chromogen. The method gives linear results up to 20 mg of uric acid/100 ml.
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