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  • Title: Quantification of urinary catecholamines, their abundant metabolites, and 5-hydroxyindoleacetic acid by high performance liquid chromatography and electrochemical detection, using a single mobile phase and uniform isocratic conditions.
    Author: Radjaipour M, Raster H, Liebich HM.
    Journal: Eur J Clin Chem Clin Biochem; 1994 Aug; 32(8):609-13. PubMed ID: 7529572.
    Abstract:
    A simple and flexible isocratic HPLC procedure was developed for the measurement of catecholamines and their abundant metabolites ( e.g. nor- metanephrine, metanephrine, methoxy-tyramine, vanillylmandelic acid, homovanillic acid) and 5-hydroxyindolacetic acid by ion-pair reversed phase chromatography on C18 columns, using a single mobile phase containing both sodium octane sulphonate and diethylamine as ion-pairing reagents.
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