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  • Title: Demonstration that thiazole-orange-positive platelets in the dog are less than 24 hours old.
    Author: Dale GL, Friese P, Hynes LA, Burstein SA.
    Journal: Blood; 1995 Apr 01; 85(7):1822-5. PubMed ID: 7535589.
    Abstract:
    Approximately 6% of dog platelets are positive for staining with thiazole orange, a dye frequently used to stain ribonucleic acid. In this report, thiazole-orange positivity is shown to mark platelets that are less than 24 hours old. Dog platelets were derivatized in vivo with N-hydroxysuccinimido biotin such that greater than 95% of all platelets were biotinylated. Newly synthesized, nonbiotinylated platelets were then monitored by flow cytometry for their ability to bind thiazole orange. After biotinylation, the percentage of biotin-negative, thiazole-orange-positive platelets increased gradually from 0.72% at 30 minutes to 5.44% at 24 hours. These data indicate that thiazole-orange staining does label newly synthesized platelets.
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