These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: The use of microwave irradiation for immunohistochemistry: a new methodological proposal.
    Author: Vitarelli E, Sippelli G, Tuccari G, Barresi G.
    Journal: Histol Histopathol; 1995 Jan; 10(1):35-8. PubMed ID: 7538834.
    Abstract:
    We developed a rapid immunohistochemical method using a microwave oven in formalin-fixed, paraffin-embedded sections from normal and pathological tissues. The strongest immunoreactivity was obtained for actin, Ca 125, CEA, pan-cytokeratin, chromogranin A, EMA, GFAP, thyroglobulin, kappa and lambda chains. In control tissues, processed with conventional immunocytochemical procedure, the reactivity was found to be qualitatively and quantitatively similar. Dako EPOS kits were also assayed with good staining intensity, shortening the original technique to 16 min. Our microwave immunohistochemical method is simple, rapid and it may be recommended for use in routine laboratories.
    [Abstract] [Full Text] [Related] [New Search]