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  • Title: Single channel behavior of recombinant beta 2 gap junction connexons reconstituted into planar lipid bilayers.
    Author: Buehler LK, Stauffer KA, Gilula NB, Kumar NM.
    Journal: Biophys J; 1995 May; 68(5):1767-75. PubMed ID: 7542035.
    Abstract:
    The beta 2 gap junction protein (Cx26) was expressed in an insect cell line by infection with a baculovirus vector containing the rat beta 2 cDNA. Isolated beta 2 gap junction connexons were reconstituted into planar lipid bilayers. Single channel activity was observed with a unitary conductance of 35-45 pS in 200 mM KCl. Channels with conductance values of 60 pS and 90-110 pS also coexisted with the lower conducting channel suggesting that there are channels with different conductance properties within a population of connexons. Channel activity was observed at voltages of up to 150 mV. Furthermore, the characterization of these channel properties from the beta 2 connexons that were generated by this heterologous expression system has provided the basis for identifying an endogenous beta 2 connexon channel in material reconstituted from native rat liver gap junctions.
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