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  • Title: Enolase and the arsonomethyl analogue of 2-phosphoglycerate.
    Author: Chawla S, Dixon HB.
    Journal: J Enzyme Inhib; 1995; 8(4):255-9. PubMed ID: 7542322.
    Abstract:
    (RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a Km of 6.5 mM (for 2-phospho-D-glycerate Km = 0.08 mM). The catalytic constant of the enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.
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