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  • Title: Presence of an additional PstI fragment in intron 1 of the chicken growth hormone-encoding gene.
    Author: Mou L, Liu N, Zadworny D, Chalifour L, Kuhnlein U.
    Journal: Gene; 1995 Jul 28; 160(2):313-4. PubMed ID: 7543870.
    Abstract:
    A previously unreported 196-bp PstI fragment was found in intron 1 of the gene encoding chicken growth hormone (cGH) when a PCR assay for an MspI restriction fragment length polymorphism was established. A pair of PCR primers was designed according to the published cGH sequence and used to amplify a fragment which contained two MspI sites, one polymorphic and another non-polymorphic. However, amplification of genomic DNA from two strains of meat-type chickens and three strains of White Leghorn chickens yielded a PCR product which was about 200 bp larger than expected. The fragment from one of the meat-type chickens was subcloned into the vector pCR-Script SK+, and sequenced. It revealed the presence of an extra fragment of 196 bp which was flanked by the PstI sites and occurred at nt +308 of the previously reported cGH sequence.
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