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  • Title: Gene expression and protein distribution of inter-alpha-trypsin inhibitor in three human hepatoma cell lines.
    Author: Héron A, Borghi H, Callé A, Bourguignon J, Diarra-Mehrpour M, Martin JP, Sesboüé R.
    Journal: Cell Biol Int; 1995 Jul; 19(7):593-602. PubMed ID: 7550067.
    Abstract:
    In standard culture conditions, three human hepatoma cell lines, Hep3B, PLC/PRF/5 and HepG2, were characterised by a predominant transcription of only two (H2 and L) among the four genes involved in the synthesis of inter-alpha-trypsin inhibitor (ITI)-related proteins. Pulse-chase experiments followed by immuno-precipitation with specific anti-L and anti-H ITI antisera showed that the proteins synthesised displayed a restricted L and/or H2 antigenic reactivity. Furthermore, while Hep3B and PLC/PRF/5 lines only synthesised ITI precursors (mainly the L-form), HepG2 cells were able to secrete an ITI-like protein. Immunocytochemical analyses substantiated these results with uneven distribution of heavy and light-chain polypeptide reactivity among the cells. The use of hepatoma cell models for the study of protein synthesis and assembly must therefore be considered cautiously.
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