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  • Title: Solid-phase extraction of nicotine and its metabolites for high-performance liquid chromatographic determination in urine.
    Author: Zuccaro P, Altieri I, Rosa M, Passa AR, Pichini S, Pacifici R.
    Journal: J Chromatogr B Biomed Appl; 1995 Jun 09; 668(1):187-8. PubMed ID: 7550977.
    Abstract:
    A solid-phase extraction, using Extrelut-1 glass columns, has been applied to urine samples of both passive and active smokers for high-performance liquid chromatographic determination of nicotine and its metabolites cotinine and trans-3'-hydroxycotinine. Chromatography was performed using a reversed-phase LC8DB column and a mobile phase consisting of water-acetonitrile (80:9, v/v) containing 5 ml triethylamine, 670 mg/l sodium heptanesulphonate, and 0.034 M each of K2HPO4 and citric acid (pH 4.4), at a flow-rate of 1.6 ml/min. The results obtained indicate that solid-phase extraction is a reliable and quick procedure which can be applied also to other nicotine metabolites.
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