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  • Title: [Exploration on experimental conditions of dot-ELISA for Trichomonas vaginalis using intact parasite antigen-monoclonal antibody detecting system].
    Author: Yu C, Gao X, Zhu Y.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 1995; 13(2):138-41. PubMed ID: 7554165.
    Abstract:
    This paper deals with the effect of different experimental conditions including the number of parasite, blocking fluid, incubation time and different types of antigens on dot-ELISA for Trichomonas vaginalis. Five microlitres of freshly washed intact worms at a concentration of 7.7 x 10(5)-3 x 10(8) cells/ml were added onto nitrocellulose membrane blocked with 1% BSA, 10% bovine serum or milk power (0.1%, 0.25%, 0.5%, 5%). Dot-ELISA using nitrocellulose membrane containing whole cell antigen 2.5 x 10(7) cells/ml, blocked by 0.5% defatted milk power at 37 degrees C for one hour before the incubation in McAb, incubated at 37 degrees C for one hour in McAb and SPA respectively was found to give more promising results. The whole cell antigen could be preserved for four months at 4 degrees C and -20 degrees C.
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