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  • Title: RNA duplex unwinding activity of alfalfa mosaic virus RNA-dependent RNA polymerase.
    Author: de Graaff M, Houwing CJ, Lukács N, Jaspars EM.
    Journal: FEBS Lett; 1995 Sep 11; 371(3):219-22. PubMed ID: 7556595.
    Abstract:
    An RNA-dependent RNA polymerase (RdRp) purified from alfalfa mosaic virus-infected tobacco is capable of synthesizing in vitro full-size RNAs of minus and plus polarities. However, the enzyme is not able to perform a complete replication cycle in vitro. The products were found to be completely base-paired to their templates. The enzyme was able to use double-stranded RNA as a template for RNA synthesis if it could initiate from a single-stranded promoter. The inability (of most) of our enzyme preparations to create a single-stranded initiation site could explain why they could not perform a complete replication cycle in vitro. This is the first report on duplex RNA unwinding activities by a plant viral RdRp.
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