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  • Title: Primer extension analysis of Streptococcus mutans promoter structures.
    Author: Smorawinska M, Kuramitsu HK.
    Journal: Oral Microbiol Immunol; 1995 Jun; 10(3):188-92. PubMed ID: 7567069.
    Abstract:
    To characterize the promoter structures of Streptococcus mutans genes, primer extension analysis was carried out with 4 genes previously isolated in this laboratory. An analysis of the transcription start sites for the gtfB, gtfC, gtfD and ftf genes revealed that each transcript was initiated at a purine residue. Based on these sites, the -10 and -35 sequences for each transcript were deduced and compared. For all 4 transcripts, the -10, but not the -35, sequences were homologous to the Escherichia coli promoter consensus sequences.
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