These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Quantitation of R- and S-propafenone and of the main metabolite in plasma.
    Author: Böhm R, Ellrich R, Koytchev R.
    Journal: Pharmazie; 1995 Aug; 50(8):542-5. PubMed ID: 7568317.
    Abstract:
    Several methods for the determination of racemic propafenone or its enantiomers as well of the main metabolite R,S-5-hydroxypropafenone are known from the literature. The method described here enables the simple simultaneous quantification of R- and S-propafenone and of R,S-5-hydroxypropafenone in human plasma. The method is based on an HPLC separation using a Chiralpak AD column. High recovery rates (80-95%) were achieved by means of a liquid-liquid-extraction at pH 11 with dichloromethane as solvent. The separation on the chiral carrier were carried out with n-hexane/2-propanol; the addition of diethylamine is useful. The obtained capacity factors are k' = 2.36 for R-propafenone and k' = 3.82 for S-propafenone. R,S-propanolol and R,S-metoprolol were used as internal standards. The method described can be used for pharmacokinetic trials in man with the following limits of quantitation: 10 ng/ml for R- and S-propafenone and 20 ng/ml for R,S-5-hydroxypropafenone.
    [Abstract] [Full Text] [Related] [New Search]