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  • Title: Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416.
    Author: Li K, West TP.
    Journal: Lett Appl Microbiol; 1995 Nov; 21(5):336-9. PubMed ID: 7576529.
    Abstract:
    Two uracil auxotrophs of the phytopathogen Burkolderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D-cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'-monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1:5-fold indicating that these pathway enzymes may be repressible by a uracil-related compound in B. cepacia. Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.
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