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  • Title: Which nutrient supplements for optimal HIV-1 production by cultured human lymphocytes?
    Author: Landureau JC, Achour A, Fouchard M, M'Bika JP, Mazière JC, Zagury D.
    Journal: Cell Mol Biol (Noisy-le-grand); 1995 May; 41(3):423-30. PubMed ID: 7580837.
    Abstract:
    Under standard conditions, all established cell-lines of HIV-1-infected lymphocytes are cultured in a medium containing a high percentage of serum. We have found that whilst partial or total serum depletion impedes their cell growth, it dramatically increases their virus production. Serial subcultures in serum-depleted medium are necessary to insure permanent cell growth and a relatively high reverse transcriptase activity. Following optimization of culture conditions, both HIV-infected and uninfected H9 cells have been regularly grown in a new protein-free medium. Such chemically defined culture conditions were required to detect the effect of minor components, such as vitamins, on host cell-virus interactions.
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