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  • Title: Localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells.
    Author: Mahon P, Beattie J, Glover LA, Hesketh J.
    Journal: FEBS Lett; 1995 Oct 02; 373(1):76-80. PubMed ID: 7589438.
    Abstract:
    The localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells was investigated using two approaches, namely Northern hybridisation of total RNA extracted from free, cytoskeletal-bound and membrane-bound polysomes isolated by a sequential detergent/salt extraction procedure and in situ hybridisation. The cytoskeletal-bound polysomes were enriched in metallothionein-I (MT-I) and c-myc mRNAs but showed a significantly lower enrichment in MT-II mRNA. These findings indicate that the MT-I mRNA is localised to the cytoskeleton during translation. In situ hybridisation using a biotin-labelled oligonucleotide probe revealed a predominantly perinuclear localisation for the MT-I mRNA.
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