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  • Title: Phage-mediated conjugation in staphylococci as a means of transfer of drug-resistance.
    Author: Mitra M, Mukherjee M, Gupta MS, Chakrabarty AN, Dastidar SG.
    Journal: Indian J Exp Biol; 1995 Jul; 33(7):505-8. PubMed ID: 7590959.
    Abstract:
    Penicillin resistance plasmid was transferred from Staphylococcus aureus B4 (PcrKms, donor) to S. aureus ML351 (PcsKmr, recipient) by co-cultivation of the donor with the recipient in nutrient broth with or without the modifying effects of CaCl2 or sodium dodecyl sulfate. It was found that the transfer of drug-resistance occurred maximally between 6 and 18 hr postinoculation; however, addition of DNase (200 micrograms/ml) could totally prevent such a transfer up to 6 hr and significantly reduce it thereafter. Cell-free filtrate of the donor culture when mixed with the recipient was ineffective in bringing about the transfer of Pcr.
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