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  • Title: Instability of Rickettsia prowazekii RNA polymerase-promoter complexes.
    Author: Aniskovitch LP, Winkler HH.
    Journal: J Bacteriol; 1995 Nov; 177(21):6301-3. PubMed ID: 7592400.
    Abstract:
    The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.
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