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  • Title: Production, characterisation and applicability of monoclonal antibodies to European eel (Anguilla anguilla L., 1758) immunoglobulin.
    Author: van der Heijden MH, Rooijakkers JB, Booms GH, Rombout JH, Boon JH.
    Journal: Vet Immunol Immunopathol; 1995 Mar; 45(1-2):151-64. PubMed ID: 7604532.
    Abstract:
    Monoclonal antibodies (mAbs) to European eel (Anguilla anguilla L., 1758) immunoglobulin (Ig) were produced, characterised and tested for applicability in a number of immuno(cyto)chemical assays. The selected mAbs, WEI 1 and WEI 2, were specifically reactive with Ig heavy and light chain, respectively. WEI 1 appeared to react with all or nearly all Ig molecules, B cells and plasma cells. WEI 2 was reactive with a subpopulation of those cells, indicating that European eel possesses at least two antigenically different light chain types. Both mAbs could be used for detection of antigen-specific antibodies in plasma by means of an enzyme-linked immunosorbent assay (ELISA).
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