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  • Title: Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes.
    Author: Roessner CA, Spencer JB, Ozaki S, Min C, Atshaves BP, Nayar P, Anousis N, Stolowich NJ, Holderman MT, Scott AI.
    Journal: Protein Expr Purif; 1995 Apr; 6(2):155-63. PubMed ID: 7606163.
    Abstract:
    The first 12 enzymes involved in the biosynthesis of vitamin B12 from its five-carbon precursor, aminolevulinic acid, have been overexpressed in recombinant strains of Escherichia coli. The activity of each enzyme has been demonstrated by the biosynthesis of hydrogenobyrinic acid from aminolevulinic acid.
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