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  • Title: Purification and cloning of DNA fragments fractionated on agarose gels.
    Author: Griffin HG, Gasson MJ.
    Journal: Mol Biotechnol; 1995 Apr; 3(2):135-8. PubMed ID: 7620974.
    Abstract:
    Purification of DNA fragments from acrylamide or agarose gels is a commonly used technique in the molecular biology laboratory. This article describes a rapid, efficient, and inexpensive method of purifying DNA fractions from an agarose gel. The purified DNA is suitable for use in a wide range of applications including ligation using DNA ligase. The procedure uses standard high-melting-temperature agarose and normal TBE electrophoresis buffer. In addition, the protocol does not involve the use of highly toxic organic solvents such as phenol.
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