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  • Title: Establishment of a rat hepatoma cell line which has ornithine carbamoyltransferase activity and grows continuously in arginine-deprived medium.
    Author: Niwa A, Yamamoto K, Yasumura Y.
    Journal: J Cell Physiol; 1979 Jan; 98(1):177-84. PubMed ID: 762194.
    Abstract:
    The epithelial cell line, H4-II-E derived from Reuber hepatoma H35 has no significant activity of ornithine carbamoyltransferase (OCT, EC 2.1.3.3) and is not able to grow in arginine-deprived medium. A multi-step selection procedure is described which selects from HR-II-E populations, cells with OCT activity which can grow in arginine-deficient, ornithine-supplemented media.
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