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Title: Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA. Author: Drew PD, Nagle JW, Canning RD, Ozato K, Biddison WE, Becker KG. Journal: Gene; 1995 Jul 04; 159(2):215-8. PubMed ID: 7622052. Abstract: We report here the nucleotide sequence of a clone, C2H2-34.10, isolated from a human brain cDNA library using degenerate oligodeoxyribonucleotide hybridization. C2H2-34.10 has extensive homology to the Xenopus laevis 5S DNA/RNA-binding protein, TFIIIA. The deduced amino acid (aa) sequence of the human clone gives a protein of 363 aa with identity to TFIIIA from both X. laevis (57%) and Rana pipiens (59%). This human clone contains nine C2H2-type zinc fingers like frog TFIIIA. Northern blot analysis indicates that the C2H2-34.10 RNA is expressed in human ovary, as well as human neuronal cell lines.[Abstract] [Full Text] [Related] [New Search]