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  • Title: Identification of a new member of the human eIF-5A gene family.
    Author: Koettnitz K, Wöhl T, Kappel B, Lottspeich F, Hauber J, Bevec D.
    Journal: Gene; 1995 Jul 04; 159(2):283-4. PubMed ID: 7622067.
    Abstract:
    Using an oligodeoxynucleotide generated by rapid PCR amplification of 5'-cDNA ends (5'-RACE) as a detection probe, we have isolated a new genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A). Sequence analysis revealed that the eIF-5A coding region is identical to the corresponding cDNA but interrupted by three introns. In a plasmid shuffle experiment we show functional replacement of the essential homologous gene in Saccharomyces cerevisiae by this human eIF-5A.
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