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  • Title: Effects of antisense oligonucleotides to the cardiac Na+/Ca2+ exchanger on cultured cardiac myocytes.
    Author: Takahashi K, Bland KS, Islam S, Michaelis ML.
    Journal: Biochem Biophys Res Commun; 1995 Jul 17; 212(2):524-30. PubMed ID: 7626066.
    Abstract:
    Antisense oligodeoxynucleotides based on the sequence of the cardiac Na+/Ca2+ exchanger were used to study the effects of decreasing expression of this protein on Na(+)-dependent Ca2+ uptake activity and on the spontaneous beating rate of cultured rat cardiac myocytes. Antisense oligos reduced Na+/Ca2+ exchanger activity of myocytes approximately 30-40%, compared to sense-treated or untreated controls. Antisense treatment also increased the spontaneous beating rate of myocardial cells from an average rate of 40 beats to 69 beats per minute, compared to sense-treated or untreated controls. These results indicate that reduced expression of the Na+/Ca2+ exchanger significantly alters intracellular Ca2+ regulation leading to an enhanced spontaneous beating rate and, presumably, a shortened duration of the cardiac cycle in developing myocytes.
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