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  • Title: Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.
    Author: Vonstein V, Johnson SP, Yu H, Casadaban MJ, Pagratis NC, Weber JM, Demirjian DC.
    Journal: J Bacteriol; 1995 Aug; 177(15):4540-3. PubMed ID: 7635839.
    Abstract:
    Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.
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