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  • Title: Improved method for polyamine determination in TMV, a rod-shaped virus.
    Author: Torrigiani P, Rabiti AL, Betti L, Marani F, Brizzi M, Bagni N, Canova A.
    Journal: J Virol Methods; 1995 May; 53(1):157-63. PubMed ID: 7635924.
    Abstract:
    Polyamines were measured in viruses using different techniques. An improved method of polyamine analysis is reported for tobacco mosaic virus (TMV), a rod-shaped virus (95% protein and 5% RNA), based on HPLC of sonicated PCA-treated highly purified virus suspensions. This method allowed higher and more reliable recovery of TMV polyamines (putrescine, spermidine and spermine) when compared to the HPLC of non-sonicated samples and to thin layer chromatography. It is suggested that sonication acts on PCA-precipitated protein aggregates causing the release of trapped polyamine molecules.
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