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  • Title: Membrane permeability and sensitivity to lethal heat are affected by lexA and recA mutations in Escherichia coli K12.
    Author: Lage C, Leitão AC.
    Journal: Braz J Med Biol Res; 1994 Oct; 27(10):2383-9. PubMed ID: 7640628.
    Abstract:
    Membrane permeability was evaluated in several SOS-deficient strains. Great heat sensitivity was observed in all the lexA (Ind-) strains, which was associated to an increase in membrane permeability (up to 120% increase above the wild-type control), as assayed by the crystal violet (CV) growth inhibition. After irradiation with a single UV dose (75 J.m-2 delivered to wild-type and 2 J.m-2 to the lexA3 strain), survival was followed by plating cells in both nutrient and membrane permeability-selective (nutrient + CV) media and a great lethality due to CV was observed in a lexA mutant, which appeared to be about 100 times more sensitive to CV compared to its wild-type parent strain. The decreased membrane integrity found in the lexA-deficient strains suggests that LexA protein and/or LexA-repressed genes may interact with the bacterial membrane, which could be the location of SOS events.
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