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  • Title: Study of the vesicles released during conservation of red cells.
    Author: Ghailani N, Guillemin C, Vigneron C.
    Journal: Nouv Rev Fr Hematol (1978); 1995; 37(2):141-7. PubMed ID: 7644352.
    Abstract:
    The purpose of this study was to determine the protein composition of the vesicles released from senescent erythrocytes and its variation during their experimental conservation at +4 degrees C in citrate phosphate dextrose (CPD), over a period of 8 weeks. Techniques employed included electron microscopy, SDS-PAGE and immunoblotting. Electron microscopic observation of senescent erythrocytes showed the simultaneous release of one or several vesicles of varying size and shape, while close examination of individual vesicles revealed a slack membrane structure and the presence of band 3 protein. SDS-PAGE and immunoblotting showed the vesicles to be composed mainly of band 3 and its breakdown products and to be lacking in spectrin. Use of specific antibodies demonstrated the presence of free haemoglobin, immunoglobulin G (IgG) and fragment C3b of complement. During conservation for 8 weeks, the concentration of band 3 protein decreased, while the concentrations of IgG and C3b increased and there was no apparent variation in haemoglobin levels.
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