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  • Title: Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.
    Author: Wypijewski KJ, Musiaø WG, Misztal L, Pater B, Malinowski T, Augustyniak J.
    Journal: Acta Biochim Pol; 1995; 42(1):97-101. PubMed ID: 7653168.
    Abstract:
    Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.
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