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  • Title: [Purification of enolase and phosphoglycerate mutase from human brain and formation of a bienzymatic complex from them].
    Author: Nazarian KB, Simonian SZ, Kazarian BA, Perez P, Climent F.
    Journal: Biokhimiia; 1995 May; 60(5):746-53. PubMed ID: 7662801.
    Abstract:
    Neuron-specific enolase and phosphoglycerate mutase with specific activities of 106 and 215 U/mg, respectively, have been purified from human brain. Hydrophobic chromatography for enolase and blue Sepharose affinity chromatography for phosphoglycerate mutase were used as the last steps of purification. A heterobifunctional complex with fully preserved enolase and phosphoglycerate mutase activities was synthesized with the use of a bifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. Autoantibodies to the conjugate will be used for identifying the bienzymatic complex in vivo.
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