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  • Title: Glucose and free fatty acid modulation of growth hormone and luteinizing hormone secretion by cultured porcine pituitary cells.
    Author: Barb CR, Kraeling RR, Rampacek GB.
    Journal: J Anim Sci; 1995 May; 73(5):1416-23. PubMed ID: 7665372.
    Abstract:
    The influence of glucose and free fatty acids (FFA) on LH and GH secretion from porcine pituitary cells was examined in culture. Cells were challenged with 10(-9), 10(-8), or 10(-7) M GnRH; 10(-9), 10(-8), or 10(-7) M [Ala15]-h growth hormone-releasing factor-(1-29)NH2 (GRF); 10(-3) or 10(-5) M forskolin; glucose (100, 300, or 600 mg/dL); and oleic or linoleic acid (10(-11) to 10(-5) M) individually or in combinations of glucose, oleic, and linoleic acid with GnRH, GRF, or forskolin. Relative to control (medium alone), GnRH increased (P < .003) LH secretion. Glucose at 100 mg/dL enhanced (P < .05) basal LH release, whereas the 600-mg/dL dose suppressed (P < .05) LH response to GnRH. Except for 10(-7) M, all doses of linoleic acid increased (P < .05) basal LH release, whereas only 10(-5) M linoleic acid suppressed (P < .02) LH response to GnRH. All doses of oleic acid enhanced (P < .05) basal LH release and suppressed (P < .03) the LH response to GnRH. In contrast, neither FFA suppressed the forskolin-induced LH secretion compared to forskolin alone. Growth hormone-releasing factor stimulated (P < .02) GH release in a dose-dependent manner. Glucose did not alter basal GH release but suppressed (P < .01) GRF-induced GH release. Only 300 mg/dL of glucose suppressed (P < .05) forskolin-induced GH release.(ABSTRACT TRUNCATED AT 250 WORDS)
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