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  • Title: The interaction of RepC initiator with iterons in the replication of the broad host-range plasmid RSF1010.
    Author: Miao DM, Sakai H, Okamoto S, Tanaka K, Okuda M, Honda Y, Komano T, Bagdasarian M.
    Journal: Nucleic Acids Res; 1995 Aug 25; 23(16):3295-300. PubMed ID: 7667106.
    Abstract:
    The replication origin of the broad host-range plasmid RSF1010 contains 3.5 copies of a 20mer iteron sequence that bind specifically to the plasmid-encoded initiator, RepC. Here we demonstrated that even a single iteron was bent upon binding of RepC. Moreover, the bending angle seems to become larger along with the increment of the number of iterons. In a mutational analysis of the iteron sequence, we isolated seven kinds of base-substitution mutants of iterons, and estimated the replication activity of these mutants in vivo. We found that each of the subsections in the 20mer iteron sequence made a distinct contribution to the initiation of RSF1010 DNA replication. With the binding assay of RepC and mutated iterons in vitro, we found that the formation of a productive RepC-iteron complex was required for the initiation of plasmid DNA replication.
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